Streptomyces lavendulaeProtease Inhibitor: Purification, Gene Overexpression, and 3-Dimensional Structure
نویسندگان
چکیده
منابع مشابه
THE GENE CLONING, OVEREXPRESSION, PURIFICATION, AND CHARACTERIZATION OF DIBENZOTHIOPHENE MONOOXYGENASE AND DESULFINASE FROM GORDONIA ALKANIVORANS RIPI90A
The biodesulfurization (BDS) of sulfur compounds in fossil fuels is a process to reduce sulfur dioxide emissions that cause environmental pollution. Gordonia alkanivorans RIPI90A is able to convert dibenzothiophene, an organic sulfur compound in petroleum, to 2-hydroxybiphenyl (2-HBP) in 4S pathway. In this study, (DBT), DszA and DszB, DBT sulfone monooxygenase, and desulfinase were respectivel...
متن کاملthe gene cloning, overexpression, purification, and characterization of dibenzothiophene monooxygenase and desulfinase from gordonia alkanivorans ripi90a
the biodesulfurization (bds) of sulfur compounds in fossil fuels is a process to reduce sulfur dioxide emissions that cause environmental pollution. gordonia alkanivorans ripi90a is able to convert dibenzothiophene, an organic sulfur compound in petroleum, to 2-hydroxybiphenyl (2-hbp) in 4s pathway. in this study, (dbt), dsza and dszb, dbt sulfone monooxygenase, and desulfinase were respectivel...
متن کاملOverexpression and Purification f As
Oyerexpression of the asnA gene from dseherichia coti K-12 coding for asparagine synthetase (EC 6.3.1.1) was achieved with a plasmid, pUNAd37, a derivative of pUC18, in E. cofi. The plasmid was constructed by optimizing a DNA sequence between the promoter and the ribosome binding region. The enzyme, comprising ca. 15% of the totRl soluble protei" in the E. coti cell, was readily purified to app...
متن کاملThe vaccinia virus H5R gene encodes late gene transcription factor 4: purification, cloning, and overexpression.
The vaccinia virus late stage-specific transcription factor P3 was purified to homogeneity from HeLa cells that were infected in the presence of an inhibitor of viral DNA replication. The purified 36-kDa protein was digested with trypsin, and the peptides were analyzed by mass spectroscopy and amino-terminal sequencing. The purified factor was identified as the product of the vaccinia virus H5R...
متن کاملPurification and structure elucidation of antifungal and antibacterial activities of newly isolated Streptomyces sp. strain US80.
A new actinomycete strain, designated US80 and producing antimicrobial activities against Gram-positive and Gram-negative bacteria and fungi, was isolated from Tunisian oasis soil. Cultural characteristic studies strongly suggested that this strain belongs to the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene (1517 pb) of Streptomyces sp. strain US80 exhibited close similarity...
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ژورنال
عنوان ژورنال: Journal of Chemistry
سال: 2015
ISSN: 2090-9063,2090-9071
DOI: 10.1155/2015/963041